Fig. 1: Comparison of CeA Drd2, Drd1a, and Adora2a populations.

Expression of Dkk3, Drd2, Drd1a, and Adora2a were examined with FISH (RNA Scope, ACD Biosystems). a Schematic of amygdala compartments within the temporal lobe. b DAPI (grey). c Dkk3 (Green) is expressed in a population of BLA pyramidal neurons. d Drd1a (Red) is expressed in striatum, weakly in some BLA cells, ITC’s (especially Im), strongly in CeL and CeM, but weakly in the CeC. e Drd2 (Cyan) is expressed in striatum, CeC, CeL, but weakly in the CeM and not ITCs or BLA. f Overlay of (b–e). The dorsal CeA especially CeL expresses both Drd2 and Drd1a populations; however, these populations segregate primarily to the CeC and CeM, respectively, more ventrally g. Schematic of higher magnification anterior dorsal region of CeA. h DAPI (Gray). i Adora2a (Green) is expressed strongly CeC, CeL and dorsal CeM. j Drd1a (Red) is expressed strongly in CeL and CeM, but little expression is found in CeC. k Drd2 (Cyan) is expressed strongly in CeC, CeL and dorsal CeM. l Overlay of (h–k). Adora2a and Drd2 entirely co-express. Very few examples of co-expression between Drd1a and either Drd2 or Adora2a are found. Examples of Drd1 expressing cells are indicated by ^. Examples of Drd2/Adora2a co-expressing cells are indicated by *. Examples of rare triple labeled Drd1a/Drd2/Adora2a cells are indicated by arrow. BLA basolateral amygdala, CeC central capsular amygdala, CeL central lateral amygdala, CeM central medial amygdala, CPu caudate putamen (striatum), Main Intercalated Island: Im. Staining was examined across n = 5 adult male animals. Scale Bar: A-F 500 μm, G-L 50 μM