Fig. 2: Co-localization of Drd2 with Crh, Nts, Prkcd, Sst, and Tac2.

Drd2 does not strongly co-express with any markers examined in anterior CeA. Drd2 moderately co-expresses with Prkcd in posterior CeC and CeL. a Density of Drd2 cell population across anterior and posterior CeA represented as a percentage of total DAPI-labeled cells. The strongest Drd2 expression is found in anterior CeC and CeL. b Quantification of Drd2 co-expression with different CeA markers at anterior and posterior positions as a percentage of total Drd2 expressing cells (CeC: two-way ANOVA with anterior vs. posterior set as row factor (F(1,79) = 13.2), p = .0005) and individual RNAs set as column factor (F(4,79) = 16.19, p < .0001). Interaction (F(4,79) = 10.56, p < .0001) and Sidak’s multiple comparisons test within row: posterior: Crh vs. Prkcd p < .0001, Nts vs. Prkcd p < .0001, Sst vs. Prkcd p < .0001, and Tac2 vs. Prkcd p < .0001; CeL two-way ANOVA with anterior vs. posterior set as row factor (F(1,74) = 2.817), p = .0975) and individual RNAs set as column factor (F(4,74) = 5.288, p < .0008). Interaction (F(4,74) = 3.901, p < .0063) and Sidak’s multiple comparisons test within row: anterior: Crh vs. Sst p < .05, posterior: Nts vs. Prkcd p < .005, Sst vs. Prkcd p < .0005, Tac2 vs. Prkcd p < .05; CeM: two-way ANOVA with anterior vs. posterior set as row factor (F(1,77) = 3.024), p = .086) and individual RNAs set as column factor (F(4,77) = 2.578, p < .05). Interaction (F(4,77) = 1.456, p = .22) and Sidak’s multiple comparisons test within row: Posterior: Crh vs. Tac2 p < .04, Tac2 vs. Prkcd p < .005, Tac2 vs Sst p < .01). c–f Map of CeA at A/P: −0.94, −1.22, −1.46, and −1.58, respectively. g–j DAPI (Gray) expression in at A/P: −0.94, −1.22, −1.46, and −1.58, respectively. k Crh (Green) is found primarily in CeL at A/P: −.84. l Prkcd (Green) is found in a isolated population at the ventral aspect of the CeC at A/P −1.22. m Prkcd (Green) is densely expressed in CeC and CeL at A/P: −1.46. n Sst (Green) is densely expressed in CeL and more diffusely in CeM at A/P: −1.58. o (Red) Tac2 is expressed in ventral CeC and CeM at A/P: −0.94. p Nts (Red) is expressed almost exclusively in CeM at A/P: −1.22. q Nts (Red) is expressed densely in CeL and diffusely in CeM at A/P: −1.46. r Crh (Red) is expressed densely in CeL and more diffusely in CeM at A/P: −1.58. s Drd2 (Cyan) is expressed strongly in CeC and CeL and more weakly in CeM at A/P: −.82. t Drd2 (Cyan) is expressed strongly in CeC and CeL and more weakly in CeM at A/P: −0.94. u, v Drd2 (Cyan) is expressed more diffusely throughout CeA at A/P −1.46 and −1.58, respectively. w–z Overlay of g–v. BLA basolateral amygdala, CeC central capsular amygdala, CeL central lateral amygdala, CeM central medial amygdala, Main Intercalated Island: Im. Staining was quantified across n = 5 male mice with a minimum of n = 8 amygdala examined. Scale bar: a–z 100 μm