Fig. 2: Dosage effect of dopamine (DA) on cell growth and genome-wide expression of 4 LCLs.
From: Transcriptomic signatures of schizophrenia revealed by dopamine perturbation in an ex vivo model
a DA effect on cell growth after 24 h of DA treatment (1 μM, 10 μM, 100 μM, and 1,000 μM). 100 μM DA started to show a significant inhibitory effect on cell growth, and most cells were dead at 1000 μM DA. Pre-treatment of the cells with the D1 receptor antagonist SCH23390 (200 nM) and/or the D2 receptor antagonist Spiperone (SP; 200 nM) for 6 h did not block the inhibitory effect of DA on cell growth. * P < 0.05 and ** P < 0.01 were derived from two-sided paired Student’s t-test. b Box plot of DA effect on genome-wide expression. 100 μM DA showed a substantially stronger effect than 1 μM DA, but pre-treatment of the cells with D1 or D2 receptor antagonists did not reverse the effect of DA. Plotted are 2999 genes that showed significant expression changes after DA stimulation (100 μM) (P < 0.05). Arrows point to the values for HMOX1
