Fig. 1: Iron treatment increases and CBD restores Caspase 3 and cleaved-PARP levels. | Translational Psychiatry

Fig. 1: Iron treatment increases and CBD restores Caspase 3 and cleaved-PARP levels.

From: Antiapoptotic effects of cannabidiol in an experimental model of cognitive decline induced by brain iron overload

Fig. 1

a Western Blotting of Caspase 3 (N = 5 rats per group) and (b) ratio of cleaved PARP to total PARP (Sorb-Veh N = 6, Sorb-CBD N = 4, Fe-Veh N = 4, Fe-CBD N = 5 rats per group) in the hippocampus of rats treated neonatally with sorbitol or iron and treated with vehicle or CBD chronically in the adulthood (3 months of age). Fifty µg of protein were separated on SDS-PAGE and probed with specific antibodies, normalized to Tubulin. Representative western blots for Caspase 3, cleaved PARP, total PARP and Tubulin are shown in the upper panel. Individual data points are plotted and mean values are presented. Statistical analysis was performed using two-way ANOVA and subsequently one-way ANOVA followed by Tukey HSD post hoc test. *p < 0.01 differences between sorbitol-vehicle (Sorb-Veh) vs. iron-vehicle (Fe-Veh); **p < 0.0001 differences between Sorb-Veh vs. Fe-Veh. #p < 0.01 difference between Fe-Veh vs. iron-CBD (Fe-CBD); ##p < 0.0001 difference between iron-vehicle vs. iron-CBD

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