Fig. 3: Iron treatment increases Cytochrome c levels.

Cytochrome c protein levels measured by ELISA assay in the hippocampus of rats treated neonatally with sorbitol or iron and treated with vehicle or CBD chronically in the adulthood (3 months of age). Statistical analysis was performed using two-way ANOVA and subsequently one-way ANOVA followed by Tukey HSD post hoc. Individual data points are plotted and mean values are presented. N = 4 rats per group. *p < 0.05 indicates a significant increase in Cytochrome c protein expression in the iron-vehicle (Fe-Veh) group compared to controls. **p ≤ 0.001 indicates a significant increase in Cytochrome c protein expression in the iron-CBD (Fe-CBD) group compared to controls