Fig. 4: Iron treatment increases and CBD restores APAF1 levels.

APAF1 protein levels measured by ELISA assay in the hippocampus of rats treated neonatally with sorbitol or iron and treated with vehicle or CBD chronically in the adulthood (3 months of age). Individual data points are plotted and mean values are represented. Sorb-Veh N = 5, Sorb-CBD N = 5, Fe-Veh N = 6, Fe-CBD N = 6 rats per group. Statistical analysis was performed using two-way ANOVA and subsequently one-way ANOVA followed by Tukey HSD post hoc test. *p < 0.0001 indicates a significant increase in APAF1 protein expression in the iron-vehicle (Fe-Veh) group compared to controls. #p < 0.0001, indicates a significant decrease in APAF1 protein expression in the iron-CBD (Fe-CBD) group compared to Fe-Veh