Fig. 3: Effect of lithium and FLUO on steady-state levels of PIns and the rate of PIns synthesis. | Translational Psychiatry

Fig. 3: Effect of lithium and FLUO on steady-state levels of PIns and the rate of PIns synthesis.

From: Lithium and fluoxetine regulate the rate of phosphoinositide synthesis in neurons: a new view of their mechanisms of action in bipolar disorder

Fig. 3

a Neurons were labelled to steady-state for 5 days (D6–11) with 3H-inositol (10 µCi/ml) in presence of 300 μM inositol and treated for 20 h with 1 mM lithium or 3 μM FLUO as indicated prior to acid extraction of the cytosol. Histograms show the cpm/well in fractions from SAX-HPLC analysis. Data are the mean and range from duplicate samples. The symbol *** indicate a highly significant (p < 0.005) increase in IP1 after lithium treatment compared with controls and the symbol (ϕ) indicates a highly significant (p < 0.005) decrease in inositol levels after FLUO compared with controls. Data shown are the mean ± SD, n = 3. b Lipids were extracted from the same neurons used for (a) and processed for chromatography of the glycerophosphoinositide head groups. Histograms show the cpm/well in fractions from SAX-HPLC analysis. Data are the mean and range from duplicate samples. The symbol * indicates a significant (p < 0.05) decrease in PIP due to lithium; similar data were obtained in three separate experiments. c Neurons from the same plating as those used to generate data in (a, b) were labelled for 1 h with 3H-inositol prior to acid extraction of the cytosol. Neurons were also treated for 20 h prior to extraction with 1 mM lithium or 3 μM FLUO as indicated. Histograms show the cpm/well in fractions from SAX-HPLC analysis. Data shown are the mean ± SD, n = 3. The symbols * and *** indicate a significant (p < 0.05) or highly significant difference (p < 0.005), respectively, with lithium treatment compared with controls. The symbol (ϕ) indicates a highly significant (p < 0.005) decrease in inositol levels of 45% with FLUO treatment compared with controls. d Lipids were extracted from the same neurons used for (c) and processed for chromatography of the glycerophosphoinositide head groups. Histograms show the cpm/well in fractions from SAX-HPLC analysis. Data shown are the mean ± SD, n = 3. The symbols ** indicate very significant differences (p < 0.01) with lithium treatment compared with controls. Similar data were obtained in three separate experiments. The symbols (ϕ) indicates a highly significant (p < 0.005) decrease in levels of PI, PIP and PIP2 with FLUO treatment compared with controls; these decreases were all equivalent to the ~45% decrease in inositol shown in (c)

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