Fig. 5: FLUO effects on the incorporation of recycled inositol into lipid PIns.

a Histograms on the left show the total uptake (cytosol plus membrane) of 3H-inositol in 1 h in control compared with 3 µM FLUO-treated neurons as indicated. Histograms on the right show the efflux from neurons in the second hour after washing away the radioactivity. The symbols indicate highly significant (p < 0.005) decreases in FLUO-treated neurons compared with controls. Data are means ± SEM, n = 4. b The ratio of radioactivity in the membranes to that in the cytosol after 1 h compared with the ratio after 2 h. The same neurons generated the left and right histogram pairs in both (a) and (b). The increase in the ratio in the second hour for FLUO-treated neurons compared with controls was highly significant (p < 0.005) as indicated ***