Fig. 2: Real-time PCR validation of the expression of glucocorticoid receptor pathway genes in three mouse models.

a Real-time PCR validation of the 23 differentially expressed glucocorticoid receptor pathway genes detected by the microarray analysis between germ-free (GF) mice (n = 8) and specific pathogen-free (SPF) mice (n = 8). b, c The six significantly expressed genes detected by PCR validation in GF mice were verified in the hippocampus of E. coli LPS-treated mice (LPS, n = 8; control, n = 8) b and of “depression microbiota” recipient mice (depression, n = 8; Healthy, n = 8) c. Data represent the mean ± SEM. * (p < 0.05, FDR < 0.1), ** (p < 0.01, FDR < 0.1)