Fig. 1: Breeding design used to generate F1, F2, and F3 progeny of HFD and chow fed dams.

Female mice (F0) were fed either HFD or chow diet for 3 weeks prior to mating, 3 weeks during gestation and 3 weeks during lactation to obtain F1 HFD and F1 CTR offspring respectively. To generate F2 offspring through paternal lineage, F1 HFD and F1 CTR males were mated with naïve females. All pregnant dams were on chow diet throughout their pregnancy and lactation. F2 HFD and F2 CTR males were mated with naïve females to obtain F3 offspring. F1, F2, and F3 offspring from both groups were on chow diet since weaning. Colors of the mice are matched with the color codes used for the groups in subsequent graphs