Fig. 3: Additional sites measured during pyrosequencing of “ARL17A cluster” correlated significantly with 450 K sites in relation to childhood abuse.
From: Exposure to childhood abuse is associated with human sperm DNA methylation

The “ARL17A cluster” found using DMRcate is located 30 kb away from ARL17A and spans 344 bp. The 450K methylation measurements of site cg04703951 (top panel) was confirmed using pyrosequencing techniques (bottom panel). The pyrosequencing assay measured DNAm at four additional sites not represented on the 450 K array (bottom panel). SD standard deviation, DMR differentially methylated region, FDR false discovery rate