Fig. 2: Dual luciferase reporter assay for modulation of MIR579 expression by upstream genetic variation and hsa-miR-579-3p-mediated regulation of anxiety candidate gene expression. | Translational Psychiatry

Fig. 2: Dual luciferase reporter assay for modulation of MIR579 expression by upstream genetic variation and hsa-miR-579-3p-mediated regulation of anxiety candidate gene expression.

From: A functional genetic variation of SLC6A2 repressor hsa-miR-579-3p upregulates sympathetic noradrenergic processes of fear and anxiety

Fig. 2

a Data show mean relative firefly luciferase activity under a minimal promoter sequence containing the (A)- and (T)-allele of rs2910931 upstream of MIR579 as indicated. Data were normalized to same-well renilla activity and are shown as mean and standard error (n = 7 technical triplicates, *p < 0.05 using a two-sided t test). b Data show mean repression of normalized firefly luciferase upon cotransfection with hsa-miR-579-3p and ath-miR159a (negative control) as indicated. Dual luciferase reporter assays contained the 3’UTR of the corresponding receptor genes as indicated. Data were normalized to negative-control-3’UTR relative firefly activity for each microRNA as indicated (n = 5 technical triplicates, *p < 0.05, ***p < 0.005)

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