Fig. 1: NGN2 transduction efficiently and consistently converts iPSCs to mature excitatory neurons across iPSC lines.

a iPSCs were infected with NGN2 and cultured as iNs to day 21, and immunostained for neuronal markers as shown. Scale bars = 50 μm. b Table summarizing lines used, including genotype as referred to here, lines as named in prior papers^11,12, DISC1 genotype, and type of control. c RNA sequencing was performed on day 21 iNs; TPM is shown for the same neuronal markers shown in a. d Single-cell multiplex qRT-PCR (Fluidigm) of 184 cells each from WT^ex8 and MUT^ex8 lines for 187 genes. Y-axis shows background-C_T, with higher numbers representing higher expression. Genes are arranged left to right and top to bottom from most similar expression across WT^ex8 and MUT^ex8 to most divergent expression. e Heatmap of single-cell data in D along with housekeeping genes, grouped by line and genotype. Columns single cells, rows single genes