Fig. 1: Protein expression and function in the developing DLPFC.

a Sample characteristics for LC-MS proteomic data (n = 69) and a subgroup of DLPFC samples with additional transcriptome data (n = 44). b Principal component analysis on global normalized protein abundance, samples are shaded by age (39 days–49.5 years). c variancePartition linear mixed model analysis of global protein abundance identifies age as a leading trait explaining most of observed protein variability. d Weighted correlation network analysis identified five protein modules, three significantly associated with age. x-axis indicates −log₁₀ p-value significance. Module names and the number of proteins within each module are displayed right of dendrogram. e Age (x-axis) relative to module eigengene (ME) expression (y-axis) for modules M1 and M2 increasing throughout development and module M4 decreasing throughout development. Smoothing linear spline models (knot = 4) were fit for each ME across age. Seven postnatal developmental stages are color shaded and labeled (SA, school age; YA, young adult). Functional annotation of age-related modules M1 (f), M2 (g), and M4 (h) according to gene ontology biological processes (FDR < 5%). i Cell-type deconvolution on global protein abundance. Data information: In (c) HGNC symbol abbreviations; CNTN1 contactin 1, HNRNPU heterogeneous nuclear ribonucleoprotein U, CSRP1 cysteine and glycine rich protein 1, CLTB clathrin light chain b, COX7C cytochrome c oxidase subunit 7c