Fig. 2: Activation or inhibition of Gβγ subunits in the nucleus accumbens has no effect on cocaine-induced hyperlocomotion, whereas interference of Gβγ subunit binding with dopamine transporter (DAT) in the nucleus accumbens attenuates amphetamine-induced locomotor activity.
From: G protein βγ subunits play a critical role in the actions of amphetamine
a Means ± s.e.m. of distance traveled by experimental period for the scr-mSIRK-saline (n = 6), mSIRK-saline (n = 6), scr-mSIRK-cocaine (n = 6), and mSIRK-cocaine (n = 6) groups. Nucleus accumbens (NAc) infusion is the period immediately after intra-NAc infusion of scr-mSIRK, and intraperitoneal (i.p.) drug is the period immediately after saline or amphetamine injection. Habituation was performed in drug-free conditions. b Means ± s.e.m. of distance traveled by experimental period for the vehicle-saline (n = 6), gallein-saline (n = 6), vehicle-cocaine (n = 6), and gallein-cocaine (n = 6) groups. NAc infusion is the period immediately after intra-NAc infusion of vehicle of gallein, and i.p. drug is the period immediately after saline or cocaine injection. Habituation was performed in drug-free conditions. c Means ± s.e.m. of distance traveled by experimental period for the TAT-scr-DATct1-saline (n = 5), TAT-DATct1-saline (n = 5), TAT-scr-DATct1-amphetamine (n = 6), and TAT-DATct1-amphetamine (n = 5) groups. NAc infusion is the period immediately after intra-NAc infusion of TAT-scr-DATct1 or TAT-DATct1, and i.p. drug is the period immediately after saline or amphetamine injection. Habituation was performed in drug-free conditions. d Means ± s.e.m. of distance traveled by experimental period for the TAT-scr-DATct1-saline (n = 5), TAT-DATct1-saline (n = 5), TAT-scr-DATct1-cocaine (n = 5), and TAT-DATct1-cocaine (n = 5) groups. NAc infusion is the period immediately after intra-NAc infusion of TAT-scr-DATct1 or TAT-DATct1, and i.p. drug is the period immediately after saline or cocaine injection. Habituation was performed in drug-free conditions. *P < 0.05 between saline-treated and cocaine- or amphetamine-treated groups; #p < 0.05 between TAT-scr-DATct1-amphetamine and TAT-DATct1-amphetamine groups
