Fig. 3: Activation or inhibition of Gβγ subunits or Gβγ–DAT interaction alters amphetamine-induced dopamine (DA) efflux in DA neurons in culture and nucleus accumbens tissue.
From: G protein βγ subunits play a critical role in the actions of amphetamine
a Inhibition of Gβγ subunits or Gβγ–DAT interaction blunts amphetamine-induced DA efflux in DA neurons preloaded with 20 nM3[H]-DA. Data represented as percent of control conditions for neurons treated with gallein (20 μM), TAT-scr-DATct1 (20 μM), TAT-DATct1 (20 μM), or TAT-DATct1 (20 μM) plus gallein (20 μM), ±amphetamine (10 μM) (n = 5/group). b Activation of Gβγ subunits increases amphetamine-induced DA efflux in nucleus accumbens (NAc) tissue. Mean ± s.e.m. of total DA efflux for tissue treated with vehicle (n = 8), scr-mSIRK (100 μM) (n = 10), or mSIRK (100 μM) (n = 10) before and after amphetamine (10 μM). c Inhibition of Gβγ subunits reduces amphetamine-induced DA efflux. Fractional release of the total DA of tissue perfused with amphetamine alone (10 μM) compared to gallein (20 μM)+amphetamine (10 μM) (n = 5/group). d Area under the curve (AUC) data for total DA efflux of tissue perfused with amphetamine alone compared to gallein+amphetamine.*P < 0.05 between amphetamine and gallein-amphetamine in NAc tissue. **p < 0.01 between mSIRK-amphetamine and vehicle-amphetamine in NAc tissue; ##p < 0.01 between amphetamine and gallein-amphetamine in DA neurons, ###p < 0.001 between amphetamine and TAT-DATct1-amphetamine in DA neurons, ****p < 0.0001 between control and amphetamine or scr-TAT-DATct1-amphetamine in DA neurons
