Fig. 5: Inhibition of Gβγ subunits in the nucleus accumbens blunts amphetamine-induced place preference but has no effect on cocaine-induced place preference.

a Means ± s.e.m. of conditioned place preference index for the gallein-saline (n = 9), vehicle-amphetamine (n = 10), and gallein-amphetamine (n = 10) groups. Vehicle or gallein was infused directly into the nucleus accumbens (NAc) 30 min before saline or amphetamine injection (intraperitoneally (i.p.)) on days 1, 3, and 5. b Similar data as shown in a, except that vehicle or gallein was administered i.p. 30 min before saline or amphetamine injection. The group sizes were identical to those in a. Animals that received gallein, whether by intra-NAc infusion (a) or i.p. injection (b), displayed neither a place preference nor an aversion, regardless of whether they underwent amphetamine conditioning or served as gallein-only controls. c Means ± s.e.m. of the conditioned preference index for cocaine-treated animals (N = 7 for both groups). Vehicle or gallein was administered i.p. 30 min before cocaine injection (i.p.) on days 1, 3, and 5. Animals displayed a place preference for the cocaine-paired compartment, regardless of whether they were pretreated with gallein or vehicle. *P < 0.05 between group average and 0.0 (no conditioning-induced shift in preference); ^#p < 0.05 between the vehicle-amphetamine-treated group and either of the two gallein-pretreated groups