Fig. 2: Binding of α-melanocyte-stimulating hormone (α-MSH)/IgG immune complex (IC) to melanocortin 4 receptor (MC4R)-expressing cells is altered in obesity and eating disorders.
From: Immunoglobulin G modulation of the melanocortin 4 receptor signaling in obesity and eating disorders
a Representative images of hMC4R GFP+ HEK 293 cells (green) 30 min after application of DyLight 550®-labeled (red) α-MSH affinity-purified IgG from eating disorder (anorexia nervosa, n = 9; bulimia nervosa, n = 7; binge eating disorder, n = 7), obese (n = 10), and Ctrl (n = 9) preincubated or not with α-MSH. Quantification of DyLight 550®-positive spots in hMC4R+ HEK 293 cells (n = 50/group): b at the membrane; c intracellularly (cytosolic), and d ratios of cytosolic/membrane staining. Affinity kinetics properties of α-MSH/IgG IC for hMC4R + HEK 293 cells including e dissociation equilibrium constant (KD); f association rate (ka), and g dissociation rate (kd). Data are means ± s.e.m. Kruskal–Wallis test with Dunns’ post-tests (b–d, f, g) or analysis of variance with Tukey’s post-test (e), ***p < 0.001, **p < 0.01, *p < 0.05
