Fig. 3: Diagrammatic representation of the region surrounding the transcription start site of IL32.

DNA methylation levels of probe cg26724967 (a constituent of the SmPGS) are negatively correlated with IL32 gene expression levels in the Dunedin Study. Shown are the locations of all 450K array DNA methylation probes in this region (based on genome assembly hg19), with cg26724967 highlighted in red. Each of the four following rows represent the four available probesets for IL32 on the Affymetrix PrimeView array, and the blue bars indicate the magnitude of the negative correlation between each probeset and DNA methylation probe in the Dunedin Study. All DNA methylation probes in this region show high negative correlations with IL32 expression, suggesting a role in IL32 gene regulation. For illustrative purposes, patterns of H3K27Ac marks (indicative of enhancer regions) and transcription factor binding site (TFBS) data from the ENCODE project are shown at the bottom of the plot; peaks of histone modification and multiple ChIP-seq identified TFBS in this location support the hypothesis that the region is important for gene regulation