Fig. 1: Microglial density, and expression of microglial markers in total brain tissue.
From: Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
a–c The medial frontal gyrus of patients with bipolar disorder (BD) and controls was stained for microglial marker ionized calcium-binding adaptor molecule (Iba1) to analyse microglial density and morphology. a Representative pictures are shown of microglia in grey (I, III) and white (II, IV) matter of controls (I–II) and patients with BD (III–IV). b The number of microglia (microglia/mm2) was manually quantified in grey and white matter in controls (N = 12, blue dots) and patients with BD (N = 12, red circles). c The number of microglia (microglia/mm2) was automatically quantified in grey and white matter in controls (N = 12, blue dots) and patients with BD (N = 16, red circles). d–h mRNA expression of microglial markers AIF1 and TMEM119, myeloid marker ITGAM and CX3CR1 and HLA-DRA in patients with BD (N = 15) and controls (N = 16) was determined using qPCR. Gene expression was normalized to 18S ribosomal RNA (18S) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) using the ΔΔCT method. Graphs show median expression levels and non-parametric testing with Bonferroni correction for multiple testing was performed. ND = number of non-detected samples
