Fig. 3: ST8SIA3 deficiency affected the overall sialylation of the striatal glycoproteins.
From: Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D2 and adenosine A2A receptors
a, b The quantitative analysis of a glycan microarray for anti-diSia-Gal antibody S2-566 and anti-triSia antibody A2B5 immunoreactivity is shown. Images of the slide obtained from a fluorescence scan after antibody incubation. The grid contains 20 types of glycans listed in Supplementary Table 2. n = 10 for each glycans with duplicates. c, d Disialyl and trisialyl units were detected in the striatal lysate. Immunoblotting revealed a significant reduction in the levels of the diSia-Gal and triSia terminal glycotopes in the striatum of St8sia3-KO (−/−) mice compared with WT (+/+) control. n = 3 for each genotype. e, f Immunoreactivity for the oligoSia and polySia-conjugated glycoproteins was detected using an anti-oligoSia antibody 12E3 and anti-polySia antibody PSA. Similar levels of the oligo-sialylated and poly-sialylated terminal glycotopes were observed in the striatum of WT and St8sia3-KO mice. n = 3 for each genotype. ACTIN was used as an internal loading control
