Fig. 5: Validating differential responses to DEX in PTSD+ participants. | Translational Psychiatry

Fig. 5: Validating differential responses to DEX in PTSD+ participants.

From: Differential transcriptional response following glucocorticoid activation in cultured blood immune cells: a novel approach to PTSD biomarker development

Fig. 5

a A total of 363 genes were significantly and uniquely responsive following 5 nM of DEX and b a total of 118 genes were uniquely responsive following 50 nM of DEX in PTSD+ participants. c Overlap analysis of DEX stimulated genes following 5 nM and 50 nM. d Real-Time quantitative PCR (RT-qPCR) was used to validate top performing mRNA targets that were uniquely upregulated in PTSD+. For these genes, the concordance between log2 fold-change statistics was assessed using a linear regression model between RT-qPCR and RNA-sequencing results for PTSD+ (red) and PTSD- (gray) participants. e Log2 fold-changes for RT-qPCR results validate a unique transcriptional response to PTSD+ participants. Asterisks (*) indicate changes that are significantly different from vehicle and were computed using a moderated t-test.

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