Fig. 1: Reactivation of GABAA receptors from frozen human synaptoneurosomes from the DLPFC. | Translational Psychiatry

Fig. 1: Reactivation of GABAA receptors from frozen human synaptoneurosomes from the DLPFC.

From: Electrophysiological evaluation of extracellular spermine and alkaline pH on synaptic human GABAA receptors

Fig. 1

a Synaptoneurosomal preparations, isolated from the dorsolateral prefrontal cortex of four subjects with no history of psychiatric disorders, were microtransplanted into Xenopus oocytes. Native GABA receptors were activated by perfusing sequentially increasing concentrations of GABA. b Average concentration response curve for GABA, using data from 4 subjects, 3–4 oocytes each. Mean ± s.e.m., error bars are smaller than the symbols. The average EC50 for all subjects pooled was 77.9 ± 4.7 µM, nH = 1 ± 0.03 (mean ± s.e.m.). The individual EC50 for each subject was: S1 = 68.3 ± 4 µM, nH = 1 ± 0.02 (n = 4 oocytes); S2 = 80.1 ± 9 µM, nH = 1 ± 0.01 (n = 3 oocytes); S3 = 96.7 ± 3 µM, nH = 1.1 ± 0.05 (n = 4 oocytes) and S4 = 63 ± 11 µM, nH = 1.2 ± 0.1 (n = 3 oocytes). c Baclofen (100 μM), an agonist of metabotropic GABAB receptors elicit negligible responses in microtransplanted oocytes. CGP-55845 (5 μM) did not significantly affect GABA elicited currents (97.3 ± 2.1% of control; n = 16 oocytes/4 subjects) in microtransplanted oocytes

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