Fig. 1: Analysis of the exosomal sncRNAome.
From: A combined miRNA–piRNA signature to detect Alzheimer’s disease
a Exosomes isolated from human CSF were analyzed via EM (upper left panel), for fragment size by using a nanosight instrument (right panel) and via immunoblot for exosomal marker proteins (lower panel). b Electropherogram showing the profile of RNA isolated from exosomes. c Electropherogram showing the profile of RNA isolated from exosome-free CSF. d Electropherogram showing the profile of RNA isolated from lysed exosomes treated with DNAase (left) and RNAase (right). e Left panel: Pie chart showing the distribution of small noncoding RNAs in human CSF exosomes. Pie chart on the top right shows the genomic distribution of the human piRNAome for comparison. The lower right pie chart shows genomic annotation of the human CSF exosomal piRNAome. Note that in contrast to the entire piRNAome (upper right pie chart), the majority of piRNAs reside in the first exon of coding genes. f Top 5 expressed miRNAs (blue) and piRNAs (red) in human CSF exosomes. g Heatmap showing expression values of the 3-p and 5-p arms of all miRNAs detected in human CSF and in the human cortex (Brodmann Area 9). h Graphs showing Pearson correlation between miRNA (two left panels) and piRNA (two right panels) expression values of hippocampal and cortical neurons vs. the corresponding miRNA and piRNA expression in exosomes released from these cells
