Fig. 4: Similar myelin pattern and thickness in adolescent and adult B6 and BTBR frontal brain.

a At P35, the pattern of MBP immunofluorescent signal was similar in the frontal brain between male B6 and BTBR mice. n = 4 mice for each strain. b Relative expression levels of striatal MBP and PLP proteins were also similar between B6 and BTBR adult male animals at 8–10 weeks old. c Schematic indicating the locations and regions where tissue was dissected (boxes), and the planes of tissue sectioning (dotted lines) for EM analysis. d Electron microscopy showed typical myelination pattern and myelin micro structure in corpus callosum (cc) and striatum (CP) from both adult male BTBR and B6 brains. Images of both lower (upper panels) and higher (lower panels) magnifications are shown. e The g-ratios of axons in both brain regions were similar between the B6 and BTBR mice. Totally, 316 axons in corpus callosum and 280 axons in striatum from four B6 mice, and 346 axons in corpus callosum and 291 axons in striatum from four BTBR mice were analyzed