Table 1 Methionine synthase activity in CHO cell homogenates after treatment of intact cells with dopamine or the D4R antagonist L-745870
From: Methylation-related metabolic effects of D4 dopamine receptor expression and activation
Cell type | Treatment | MS activity (pmol/min/mg) | %WT basal |
|---|---|---|---|
WT CHO | Basal | 205 ± 12 | 100 |
Dopamine (10 µM; 30 min) | 208 ± 18 | 101 | |
L-745870 (100 nM; 60 min) | 201 ± 7 | 98 | |
Dopamine + L-745870 | 216 ± 15 | 105 | |
D4.2 CHO | Basal | 20.5 ± 1.8a | 10 |
Dopamine | 38.5 ± 3.3b | 19 | |
L-745870 | 6.6 ± 0.7b | 3 | |
Dopamine + L-745870 | 6.9 ± 0.6b | 3 | |
D4.4 CHO | Basal | 21.2 ± 0.5a | 10 |
Dopamine | 48.7 ± 4.6b | 24 | |
L-745870 | 6.5 ± 0.5b | 3 | |
Dopamine + L-745870 | 6.7 ± 0.7b | 3 | |
D4.7 CHO | Basal | 18.8 ± 0.8a | 9 |
Dopamine | 56.6 ± 4.3b,c | 28 | |
L-745870 | 6.4 ± 0.3b | 3 | |
Dopamine + L-745870 | 6.5 ± 0.5b | 3 |
