Fig. 4: Morphological analysis of Case #5 (ARHGAP10 CNV/SNV) neurons.
From: ARHGAP10, which encodes Rho GTPase-activating protein 10, is a novel gene for schizophrenia risk
a–f In vitro morphological analysis of human neurons derived from iPSCs; Control #1 (a), Control #2 (c), Case #5, clone #1 (b), Case #5, clone #4 (d). e Average number of branches on the primary neurites (Control #1, 0.514 ± 0.086, n = 72; Control #2, 0.487 ± 0.087, n = 78; Case #5, clone #1, 0.252 ± 0.040, n = 147; Case #5, clone #4, 0.209 ± 0.054, n = 67; t-test P values: Control #1 vs Case #5, clone #1, p = 0.0068; Control #1 vs Case #5, clone #4, p = 0.0034; Control #2 vs Case #5, clone #1, p = 0.0153; Control #2 vs Case #5, clone #4, p = 0.0076). f Average length of primary neurites (Control #1, 116.3 ± 4.29 μm, n = 382; Control #2, 126.2 ± 3.81 μm, n = 562; Case #5, clone #1, 59.0 ± 1.88 μm, n = 575; Case #5, clone #4, 65.7 ± 3.82 μm, n = 252; t-test P values: Control #1 vs Case #5, clone #1, p = 0.0025; Control #1 vs Case #5, clone #4, p = 0.0023; Control #2 vs Case #5, clone #1, p = 0.0002; Control #2 vs Case #5, clone #4, p < 0.0001). g Two iPS cell clones derived from healthy controls and two iPS cells derived from Case #5 were differentiated into neurons, dispersed, and cultured on a plate. Time-lapse observation was then performed every 12 h and imaged for 60 h after seeding at three concentrations (0, 1, and 10 μM) of the Rho-kinase inhibitor, Y-27632. h A graph obtained by analyzing the image data of (g) the automatic tracking with NeuroTracker in IncuCyte Zoom. Culture time was plotted on the horizontal axis, while neurite outgrowth was plotted on the vertical axis. Statistical analyses are shown in Supplementary Fig. 7b. Values indicate the mean ± S.E.M.
