Fig. 5: Knock down of Nedd4l rescues CSDS-induced depression.
From: Nedd4l downregulation of NRG1 in the mPFC induces depression-like behaviour in CSDS mice
a Experimental timeline of mPFC bilateral injection of AAV9-EGFP or AAV9-Sh-Nedd4l viruses and behavioural studies. Downregulation of Nedd4l expression before CSDS-induced depression-like behaviours as assessed by b social interaction, c sucrose preference and d tail suspension tests, and anxiety-like behaviours as assessed by e novelty-suppressed feeding, f open field and g the elevated plus maze tests, n = 10/8/8/8. h–k Representative western blots and quantification of NRG1, Nedd4l and NRG1 ubiquitination (UBI) identified in the mPFC of non-stressed (CTR) mice, and mice subjected to CSDS after bilateral injection of AAV9-EGFP or AAV9-Sh-Nedd4l viruses, n = 3 per group. l, m Golgi staining was performed to evaluate the changes of dendritic spines in the mPFC between CTR and CSDS mice after injection of AAV9-EGFP or AAV9-Sh-Nedd4l viruses. At least ten neurons from three mice per group were analysed. Scale bars, 10 μm. p-Value significance is calculated from a one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data represent mean ± SEM.
