Fig. 3: Anxiety and mood.
a The Light-dark box test revealed no difference between the wild-type and the Tal1cko mice in anxiety-like behavior. Both groups displayed similar avoidance of brightly illuminated compartment as shown by the percentage of time and distance moved in light compartment (time, F1,84 = 0.002, p = 0.96; distance, F1,84 = 0.0004, p = 0.98), N = 88 (27 M WT; 19 M KO; 24 F WT; 18 F KO). b Activity of the Tal1cko mice (total distance traveled) increased significantly during the second half of the testing period in the light-dark box (RM ANOVA genotype x time interaction F1,84 = 53.2, p < 0.0001). ***p < 0.001, between genotypes, Bonferroni’s test, N = 88 (27 M WT; 19 M KO; 24 F WT; 18 F KO). c In the forced swim test, the time of immobility in 2 min intervals was significantly reduced in the Tal1cko mice (RM ANOVA genotype F1,28 = 39.3, p < 0.0001, interaction genotype x time F2,56 = 29.1, p < 0.0001), ***p < 0.001, between genotypes, Bonferroni’s test, N = 30 (18 M WT; 12 M KO). d Stress-induced hyperthermia. The Tal1cko mice had lower basal body temperature (F1,22 = 8.5, p = 0.0081), and stress induced equal hyperthermic effect in both Tal1cko and wild-type mice (F1,22 = 0.5, p = 0.49). **p < 0.01, between genotypes, ANOVA. N = 24 (14 M WT; 10 M KO). e Sociability test. Time with the stimulus mouse was not altered between the genotypes (F1,32 = 0.007, p = 0.94). N = 36 (9 M WT; 7 M KO; 13 F WT; 7 F KO). f Prepulse inhibition of acoustic startle reflex was not different between the groups (RM ANOVA genotype F1,28 = 1.4, p = 0.25), but increased with the increasing sound pressure level of pre-pulse (RM ANOVA pre-pulse level F3,84 = 9.3, p < 0.001; genotype x pre-pulse level interaction F3,84 = 0.2, p = 0.9), N = 30 (18 M WT; 12 M KO).
