Fig. 4: Putative pyramidal cells in the somatosensory cortex have decreased GABA amplitude.
From: Full function of exon junction complex factor, Rbm8a, is critical for interneuron development
A, B There is no change in the frequency (A) or amplitude (B) of the excitatory spontaneous postsynaptic current of Nes-cre; Rbm8afl/+ mice. Spontaneous excitatory postsynaptic current recordings from putative pyramidal cells in the somatosensory cortex, Student’s t test, n = 7–12. C Representative glutamate traces from control and cKO mice. D, E There is a significant decrease in the frequency (D), but not the amplitude (E) of the spontaneous inhibitory postsynaptic current in Nes-cre; Rbm8afl/+ mice. Spontaneous inhibitory postsynaptic current recordings from putative pyramidal cells in the somatosensory cortex, *p < 0.05, n > 7, Student’s t test. F Representative GABA trace from control and cKO mice. Bar graphs represent means ± SEMs. G Western blot showed a significant reduction of GABA-A receptor β3 subunit. *p < 0.05; n = 3, Student’s t test. Bar graphs represent means ± SEMs. H Western blot showed a no significant reduction of GAD67. n = 3, Student’s t test. Bar graphs represent means ± SEMs.
