Fig. 2: Immunofluorescence staining on primary hippocampal live neurons of plasma samples from selected individuals.
From: Novel neuronal surface autoantibodies in plasma of patients with depression and anxiety
Neurons were stained with anti-MAP2 (red), autoantibodies were stained with anti-human IgG (green) and nuclei were stained with DAPI (blue). Serum from an encephalitis patient with NMDAR autoantibodies was used as a positive control (“Pos”), plasma from a healthy individual was used a negative control (“Neg”). Plasma samples from Case 2, Case 5, and Case 9 showed clear immunoreactivity to live neurons. Scale bar = 50 µm. Regions in white boxes are shown at higher magnification on the right panels; these show a speckled human IgG staining along the dendrite. Scale bar = 20 µm.
