Fig. 5: Development and maintenance of the basal forebrain cholinergic neurons were similar among genotypes; but postsynaptic defects were observed in the hippocampal neurons of the Ntrk1 KI mice. | Translational Psychiatry

Fig. 5: Development and maintenance of the basal forebrain cholinergic neurons were similar among genotypes; but postsynaptic defects were observed in the hippocampal neurons of the Ntrk1 KI mice.

From: Ntrk1 mutation co-segregating with bipolar disorder and inherited kidney disease in a multiplex family causes defects in neuronal growth and depression-like behavior in mice

Fig. 5

a Western blots of the ChAT protein using the basal forebrain tissue homogenates. N = 3 for each genotype, representative blots were shown. b Numbers and sizes of the basal forebrain cholinergic neurons, N = 3 pairs. MS medial septum, VDB ventral limb of the diagonal band of Broca; HDB horizontal limb of the diagonal band of Broca, NB nucleus of basalis. c Western blots of the pERK and ERK proteins using the basal forebrain tissue homogenates. N = 3 pairs, representative blots were shown. d Acetylcholine esterase immunohistochemistry in the hippocampal CA1 region. Bar, 250 μm. Acetylcholine esterase (AChE) immunohistochemistry in the hippocampal CA1 region. Anti-AChE antibodies (HPA019704, Sigma-Aldrich) were used. Bar, 250 μm. e pERK/ERK ratios of the hippocampus by fluorescence western blots using the tissue homogenates under saline or physostigmine administration. N = 3 pairs. *p < 0.05; †p = 0.070 (t-test, two-tailed); Error bars, mean ± s.e.m. f Total length of the dendrites in the primary hippocampal neurons. g Number of intersections in the dendrites of the primary hippocampal neurons. f, g +/+, N = 44; KI/+, N = 84; KI/KI, N = 85 neurons were counted. *p < 0.05 (One-way ANOVA followed by Fisher’s PLSD post-hoc test); Error bars, mean ± s.e.m.

Back to article page