Fig. 5: Analyses of zdhhc15 substrates and proteins involved in DA metabolism in striatum of zdhhc15-KO mice. | Translational Psychiatry

Fig. 5: Analyses of zdhhc15 substrates and proteins involved in DA metabolism in striatum of zdhhc15-KO mice.

From: Increased novelty-induced locomotion, sensitivity to amphetamine, and extracellular dopamine in striatum of Zdhhc15-deficient mice

Fig. 5

A, B Western blot analyses of DAT and TH in striatal proteins extracts from zdhhc15-KO and WT mice revealed no significant differences between the two genotypes (n = 10 animals per group, student t-test). C Levels of other proteins involved in DA signaling and degradation were similar between WT and KO mice (n = 5 animals per group; student t-test, p > 0.05). D Acyl-biotin exchange (ABE) assay was performed for quantitative analysis of protein palmitoylation in striatum from WT and zdhhc15-KO mice. Representative blot signals of inputs (left) and ABE samples (right) in striatum of zdhhc15-KO and WT mice are shown. No differences were found in the total level of proteins or in the palmitoylation levels of known DHHC15 substrates (n = 5–6 animals per group; student t-test, p > 0.05).

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