Fig. 4: FACS analysis of PFC samples from vehicle or PLX3397 treated mice. | Translational Psychiatry

Fig. 4: FACS analysis of PFC samples from vehicle or PLX3397 treated mice.

From: Essential role of microglial transforming growth factor-β1 in antidepressant actions of (R)-ketamine and the novel antidepressant TGF-β1

Fig. 4

a FACS analysis of CD11b-gated cells stained with antibody to TMEM119 in PFC samples of control mice and PLX3397 treated mice. b Iba1 and TGF-β1 expression in TMEM119+CD11b+ microglia were analyzed. Red histograms indicate control group and blue histograms indicate PLX3397 treated group. c The fluorescence intensity of both Iba1 and TGF-β1 in TMEM119+CD11b+ microglia in the PFC of PLX3397 treated mice was significantly (Iba1: P = 0.0186, TGF-β1: P = 0.0002) lower than that of control mice. d Western blot analysis of Iba1 in the PFC samples of control mice and PLX3397 treated mice. Representative bands of Western blot analysis. The expression of Iba1 in the PFC of PLX3397 treated mice was significantly (P = 0.0023) lower than that of control mice. Data are shown as mean ± SEM. (control group: n = 10, PLX group n = 9). *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page