Fig. 2: Any cafeteria diet exposure affects gut microbiome composition and alters predicted bacterial metabolic pathways. | Translational Psychiatry

Fig. 2: Any cafeteria diet exposure affects gut microbiome composition and alters predicted bacterial metabolic pathways.

From: Intermittent cafeteria diet identifies fecal microbiome changes as a predictor of spatial recognition memory impairment in female rats

Fig. 2

a Microbial species richness, b microbial species evenness, and c Shannon’s diversity following 7 weeks of diet exposure. Data are expressed as box-and-whisker plots (min, IQR, max); n = 11–12; data were analyzed by one-way ANOVA followed by Tukey-adjusted post-hoc comparisons (ap < 0.05 relative to Chow). d Non-metric multidimensional scaling (using Bray–Curtis similarity scores) on fecal microbiome samples; n = 11–12. e Operational taxonomic units (OTUs) differentially expressed between Chow, Cycle, and Cafeteria groups. Data are expressed using both DeSeq2 (log(Fold change), FDR-adjusted p-value < 0.05), and LefSe (LDA score > 2, FDR-adjusted p < 0.05) scores. The color of the circle/bar denotes the group the OTU is enriched in (open: Chow, gray: Cycle, black: Cafeteria); n = 11–12. f Ether lipid metabolism, g flavone and flavonol biosynthesis, and h flavonoid biosynthesis predicted using PICRUSt from fecal microbiome data at 7 weeks. Data are expressed as box-and-whisker plots (min, IQR, max); n = 11–12; data were analyzed by one-way ANOVA (FDR-adjusted overall p-value to account for multiple relevant pathways included in the analysis) followed by Tukey-adjusted post-hoc comparisons (ap < 0.05 relative to Chow, bp < 0.05 relative to Cycle).

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