Table 1 Body composition, tissue collection, and plasma measures.

From: Intermittent cafeteria diet identifies fecal microbiome changes as a predictor of spatial recognition memory impairment in female rats

 

Chow

Cycle

Cafeteria

Diet effect

EchoMRI (3 days prior to kill)

 Fat mass (g)

46.8 ± 4.1

73.1 ± 6.8a

126.1 ± 9.6a,b

p < 0.001

 Fat percentage (%)

14.2 ± 1.1

20.7 ± 1.4a

29.5 ± 1.4a,b

p < 0.001

 Lean mass (g)

262.6 ± 6.5

256.2 ± 5.4

277.0 ± 7.4

Tissue collection

 Final body weight (g)

329.9 ± 7.9

367.0 ± 12.0

433.4 ± 17.3a,b

p < 0.001

 Liver weight (g)

9.95 ± 0.35

11.96 ± 0.54a

12.68 ± 0.49a

p = 0.001

 Retroperitoneal fat pad weight (g)

4.81 ± 0.45

9.40 ± 1.14a

13.15 ± 0.71a,b

p < 0.001

 Ovary weight (mg)

46.0 ± 2.1

44.7 ± 1.93

50.2 ± 2.4

 Total oocytes/female

20.5 ± 1.3

17.1 ± 1.5

15.1 ± 1.8a

p = 0.024

Blood/plasma measures

 Blood glucose (mmol/L)

7.7 ± 0.3

8.3 ± 0.3

9.3 ± 0.5a

p = 0.014

 Plasma insulin (ng/mL)

0.19 ± 0.05

0.91 ± 0.20a

1.08 ± 0.22a

p = 0.002

 Plasma leptin (ng/mL)

5.69 ± 0.68

11.35 ± 1.28a

17.38 ± 1.67a,b

p < 0.001

 Plasma triglycerides (mmol/L)

1.06 ± 0.08

2.30 ± 0.34a

3.48 ± 0.49a

p = 0.001

  1. Results expressed as mean ± SEM; n = 11–12; data were analyzed by one-way ANOVA followed by Tukey-adjusted post-hoc testing (ap < 0.05 relative to Chow, bp < 0.05 relative to Cycle). Total oocytes per female were analyzed using an independent-samples Jonckheere–Terpstra test for ordered alternatives followed by non-parametric Bonferroni–Dunn post-hoc testing (ap < 0.05 relative to Chow).