Fig. 1: Experimental design.
a The exposure facility consisted of a vivarium, where animals were housed in FA or TRAP exposure chambers, and a behavioral testing suite. TRAP drawn from a freeway tunnel system in Northern California was delivered unchanged to the exposure chamber. FA exposures were created by sequentially filtering ambient air adjacent to the vivarium to remove volatile and semi-volatile components, NOx, and ultrafine and fine particulate matter. b PM2.5 and total suspended particulates (TSP) in TRAP vs. FA shown as the mean ± SD (n = 8). c Pregnant dams were transported to the facility at gestational day 14; pups were born in the facility and remained there until PND 47–51. Pups were divided into two cohorts: one that was not used for behavioral studies (yellow bar) and another that was behaviorally tested and imaged by MR (green bar).
