Fig. 3: Behavioral effects of habituation, novelty, and amphetamine in Sorcs2−/− mice.
A, B Sorcs2−/− mice are significantly more active (p < 0.0001, Student’s unpaired t-test) and C, D rear significantly more (p < 0.003, Student’s unpaired t-test) in a novel environment compared to Wt mice. E Wt and Sorcs2−/− mice show normal activity in their home-cage (p > 0.4, two-way ANOVA). F Interaction between genotype and amphetamine dose on locomotor activity (p < 0.05, two-way ANOVA) G but not rearing (p > 0.2, two-way ANOVA) in habituated mice. H Interaction between genotype and amphetamine dose on locomotor activity (p < 0.007, two-way ANOVA) I but not rearing (p > 0.08, two-way ANOVA) in non-habituated mice was observed. In non-habituated mice, a significant increase in activity was observed in Wt mice when treated with 10 mg/kg compared to the vehicle group, while the activity decreased in Sorcs2−/− mice (H, p < 0.03 for both genotypes, Student’s unpaired t-test). Data in A, C + F–I are presented as mean activity count/rearing for one hour per mouse ± S.E.M (n = 48 mice per group for A and C and 12 mice per group for F–I). Data for B and D are the corresponding data to A and C, respectively, in which activity/rearing is shown over time in 5 min intervals, Data in E are presented as mean activity count for 24 h per day per mouse ± S.E.M (n = 8 mice per group). Above the graph, it is stated if the mice were habituated or not to the test-cage prior to drug treatment. Asterisk indicates a significant difference between genotypes, while # and § indicate significant differences between the treated group and vehicle group within Wt and Sorcs2−/− mice, respectively. *, # and § p < 0.05; **, ## and §§ p < 0.01; *** and §§§ p < 0.001; ****, #### and §§§§ p < 0.0001.
