Fig. 4: Sweet solution preference test, aggressive behavior, and brain mitochondrial basal respiration of mice following in vivo rotenone-induced mild MD. | Translational Psychiatry

Fig. 4: Sweet solution preference test, aggressive behavior, and brain mitochondrial basal respiration of mice following in vivo rotenone-induced mild MD.

From: Using mitochondrial respiration inhibitors to design a novel model of bipolar disorder-like phenotype with construct, face and predictive validity

Fig. 4

Rotenone (0.75 mg/kg/day) was administered i.p. for 4 or 8 weeks. Lithium (Li) was given for the last 2 weeks of rotenone treatment. Results represent means±SEM of 12 mice/group. One result of the SSPT and one result from the basal respiration data which exceeded ±2SDs were excluded. A The effect in the SSPT. The amount of saccharine/total fluids drank was significantly increased following 4 weeks of treatment and significantly decreased following 8 weeks. Two-way ANOVA with treatment (±Rot.) and duration of treatment (4/8 weeks) as main factors: #values are of the TreatmentXDuration interaction; Fisher’s LSD post hoc test: *4/8 weeks of Rot. vs. vehicle, p < 0.02. B The effect on aggressiveness. Time spent in aggressive behavior was 1.8-fold non-significantly increased following 4 weeks of treatment and 4.6-fold significantly decreased following 8 weeks. Two-way ANOVA with treatment (±Rot.) and duration of treatment (4/8 weeks) as main factors: #values are of the TreatmentXDuration interaction. Fisher’s LSD post hoc test: *8 weeks of vehicle vs. 4 weeks of vehicle, p < 0.0009; **8 weeks rotenone vs. vehicle, p = 0.0001. C The effect on hippocampal mitochondrial basal respiration. *t-test analysis showed a significant reduction following 8 weeks of Rot. (p = 0.01). D The effect on frontal-cortex mitochondrial basal respiration. *t-test analysis showed a significant increase following 4 weeks of Rot. (p = 0.05).

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