Fig. 1: Characterization of monocyte-derived induced microglia-like cells (iMGs) by direct cytokine reprogramming.
From: Umbilical cord blood-derived microglia-like cells to model COVID-19 exposure
a Umbilical cord monocyte-derived CB-iMGs. b Adult PBMC-derived iMGs. (i) Morphology by phase contrast; immunostained images of iMG cells stained with nuclei (Hoechst) and indicated microglial markers (ii) IBA1, (iii) CX3CR1, (iv) P2RY12, and (v) PU.1. Scale bar: 30 μm. c Quantitation of positively immunostained cells as a percentage of total cells (nuclei) for indicated microglial markers. Bars indicate mean of indicated no. of cells measured, error bars indicating SEM. d Quantitation of fold increase in gene expression of indicated microglial markers normalized to input (PBMCs and CB-MNCs, respectively) after iMG reprogramming, error bars indicating SEM (n = 3 measurements).
