Fig. 2: The activity of PTEN signaling pathway is up-regulated in the PFC of CRS-treated mice, and it can be prevented by the administration of PTEN inhibitor.
From: PTEN in prefrontal cortex is essential in regulating depression-like behaviors in mice
a Diagram of experiment design and timeline. Cortical tissues from the two subregions of PFC, Cg1, and PrL were collected for Western blots. b–f One-month CRS treatment led to the increase in the levels of PTEN, p-MEK1, and p-ERK1/2 and decrease of p-AKT in the PFC, but these changes were not observed in the CRS-treated mice receiving daily injection of PTEN inhibitor during the period of days 15–30. Note that the levels of p-PTEN, ERK1/2, AKT, and MEK1 were not changed by the CRS-treatment or application of PTEN inhibitor. n = 4 in each group and data are presented as mean ± SEM. Two-way ANOVA with post hoc Tukey’s test, *p < 0.05, **p < 0.01. Cg1, cingulate cortex, area 1; CRS, chronic restraint stress; PrL, prelimbic cortex; VO-Ohpic, VO-Ohpic trihydrate.
