Fig. 5: Deposition of MG-H1 and mitochondrial proteins in mitochondria from WT and GLO1 KO cells. | Translational Psychiatry

Fig. 5: Deposition of MG-H1 and mitochondrial proteins in mitochondria from WT and GLO1 KO cells.

From: Glyoxalase I disruption and external carbonyl stress impair mitochondrial function in human induced pluripotent stem cells and derived neurons

Fig. 5

A Immunofluorescence images of MG-H1 (green) in mitochondria labeled with Mitotracker® (red) in WT-hiPSCs (scale bar: 20 µm). MG-H1 immuno-positive staining was partially localized in mitochondria. B Representative Western blot for VDAC1, HSP60, and GAPDH in lysates from whole cell and isolated mitochondria. C Western blotting for MG-H1 and VDAC1 in mitochondria isolated from WT- and GLO1 KO-hiPSCs after treatment with 50 µM MGO for 72 h. D Densitometric analysis of the Western blot shown in C. Significant increase in MG-H1 modification was observed in mitochondria from GLO1 KO-hiPSCs after treatment with 50 µM MGO. E Western blotting for MG-H1 and VDAC1 in mitochondria isolated from WT- and GLO1 KO-derived neurons after treatment with 50 µM MGO for 24 h. Mitochondria from GLO1 KO-derived neurons showed increase in MG-H1 modification upon MGO exposure. Data represent mean ± SEM. **P < 0.01; two-way ANOVA, followed by Tukey’s multiple comparison test.

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