Fig. 4: Knockdown of BRUCE represses the autophagosome-lysosome fusion and BDNF nuclear transport in HT22 cells. | Translational Psychiatry

Fig. 4: Knockdown of BRUCE represses the autophagosome-lysosome fusion and BDNF nuclear transport in HT22 cells.

From: BRUCE silencing leads to axonal dystrophy by repressing autophagosome-lysosome fusion in Alzheimer’s disease

Fig. 4

A The interaction between BRUCE and STX17 in cells determined by Co-IP assay. B The co-localization between BRUCE and STX17 detected by immunofluorescence staining (400×). C The HT22 cells treated with knocked-down BRUCE detected by Western blot analysis, *p < 0.05 compared with cells treated by sh-NC. The HT22 cells were treated with sh-NC or sh-BRUCE. D The autophagosome-lysosome fusion in cells (400×). E The BDNF nuclear transport determined by immunofluorescence staining (200×). F The protein expression of BDNF and p-CREB/CREB in cells, * p < 0.05 compared with cells treated with sh-NC. G The expression of BDNF in the nucleus of cells detected by Western blot analysis, p < 0.05 compared with cells treated with sh-NC. H The immunofluorescence staining of axons in cells detected by immunofluorescence staining, *p < 0.05 compared with cells treated with sh-NC. The experiment was repeated three times independently.

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