Fig. 5: Inhibition of miR-204 promotes the autophagosome–lysosome fusion and rescues defective axonal transport by increasing BRUCE in HT22 cells. | Translational Psychiatry

Fig. 5: Inhibition of miR-204 promotes the autophagosome–lysosome fusion and rescues defective axonal transport by increasing BRUCE in HT22 cells.

From: BRUCE silencing leads to axonal dystrophy by repressing autophagosome-lysosome fusion in Alzheimer’s disease

Fig. 5

A The miR-204 expression in the hippocampus tissues of AD mice and normal mice, *p < 0.05 compared with normal mice. B The binding site between miR-204 and BRUCE3′UTR predicted by bioinformatics analysis. C The targeting relationship between miR-204 and BRUCE identified by dual-luciferase reporter gene assay. *p < 0.05 compared with mimic-NC treatment. The cells were treated with mimic-NC, miR-204 mimic, inhibitor-NC, and miR-204 inhibitor. D The expression of miR-204 and mRNA expression of BRUCE in cells measured by RT-qPCR. E The protein expression of BRUCE in cells examined by Western blot analysis. F The fusion of autophagosome and lysosome in cells detected by immunofluorescence staining (400×). G The protein expression of BDNF and p-CREB/CREB in cells determined by Western blot analysis. H The expression of BDNF protein in the nucleus of cells determined by Western blot analysis. I The immunofluorescence staining of axons in HT22 cells detected by immunofluorescence staining. *p < 0.05 compared with cells treated with mimic-NC; #p < 0.05 compared with cells treated with inhibitor-NC. The experiment was repeated three times independently.

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