Fig. 1: Localization of TN and TX to DA neurons and striatal neurons.

A Double immunostaining of midbrain sections for TH (red) and TN or TX (green) shows TN or TX expression located in the VTA or substantia nigra. B Immunostaining in striatal medium spiny neurons shows prominent nuclear TX staining (green) in DARPP-32 positive neurons (red) (top row). TX staining of sections from mice expressing tdTomato in D1R + neurons demonstrates that TX is expressed in both D1R-positive neurons as well as in D1R-negative neurons (bottom row). Scale bar, 30 μm.