Fig. 2: Tsn KO mice display increased locomotor response to amphetamine (AMPH) but normal levels of tissue DA, TH, DAT and D2R. | Translational Psychiatry

Fig. 2: Tsn KO mice display increased locomotor response to amphetamine (AMPH) but normal levels of tissue DA, TH, DAT and D2R.

From: Elevated body fat increases amphetamine accumulation in brain: evidence from genetic and diet-induced forms of adiposity

Fig. 2

A Amphetamine-induced (2.5 mg/kg, i.p.) locomotor activity is increased in male KO mice. Locomotor activity was monitored every 5 min and arrowheads indicate the time of injections. n = 8/group. Two-way ANOVA with RM revealed a significant effect of time (p < 0.0001), a significant effect of genotype (p = 0.0095), as well as a significant interaction between time X genotype (p < 0.0001). Bonferroni post-hoc testing was done to identify individual time points that were significantly different. **p < 0.01, and ***p < 0.001. B Immunostaining shows comparable TH staining of DA neurons (left panel) and striatal terminals (right panel) in WT and translin KO mice. Scale bar, 500 μm. C Tissue DA levels in the nucleus accumbens (NAc) and striatum (STR) are similar in WT and translin KO mice. n = 5–6/group. D, E Immunoblotting shows normal TH, DAT, D2R expression levels in NAc and STR in translin KO mice. Image J was used for quantification. n = 6/group. Data are expressed as mean ± SEM. Statistical significance was assessed by Student’s t-test.

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