Fig. 2: Bilobalide inhibits STAT3-dependent inflammation responses in Aβ42-treated astrocyte culture.

A Western blot and its quantification showing STAT3 protein levels in astrocyte culture treated by 5 μM Aβ42 and bilobalide at indicated concentrations. B STAT3 luciferase reporter assay showing STAT3 transcription activity in astrocyte culture treated by 5 μM Aβ42 and bilobalide at indicated concentrations. C Quantitative real-time PCR results showing the levels of TNF-α, IL-1β, and IL-6 in the astrocyte culture treated by 5 μM Aβ42 and bilobalide at indicated concentrations. D ELISA results showing the levels of TNF-α, IL-1β, and IL-6 in the astrocyte supernatant under the treatment of 5 μM Aβ42 and bilobalide at indicated concentrations. E CCK-8 assay showing the astrocyte proliferation under the treatment of 5 μM Aβ42 and bilobalide at indicated concentrations. *P < 0.05, **P < 0.01, ***P < 0.001.