Fig. 4: The similar differentiation markers expressing in SCA3-NSCs and isogenic control SCA3-NSCs. | Translational Psychiatry

Fig. 4: The similar differentiation markers expressing in SCA3-NSCs and isogenic control SCA3-NSCs.

From: CRISPR/Cas9 mediated gene correction ameliorates abnormal phenotypes in spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cells

Fig. 4

a The scheme of neuronal differentiation protocol in vitro, the mature neurons were obtained about 60 days after differentiation. b–f Neural induction resulted in robust expression of NSCs. b NSCs markers, PAX6, NESTIN, FOXG1 (day 16) as measured by qRT-PCR (n = 3 per sample independent biological replicates), the higher expression of NSCs markers in NSCs stage compared with iPSCs stage. c–f PAX6 and NESTIN (day 16) as detected by immunocytochemistry. There was no difference in each group (P > 0.05). Scale bar: 100 µm. d, f All data were presented as mean ± SD. The data was determined by one-way ANOVA (P = 0.05) and Bonferroni post-hoc test among the groups. n = 5 images for immunofluorescence. Ctrl-NSCs: healthy control NSCs. SCA3-NSCs: SCA3 patient-derived NSCs. SCA3-C3-NSCs and SCA3-C12-NSCs: corrected SCA3-NSCs.

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