Fig. 1: Characterization of iPSC-derived astrocytes. | Translational Psychiatry

Fig. 1: Characterization of iPSC-derived astrocytes.

From: A human iPSC-astroglia neurodevelopmental model reveals divergent transcriptomic patterns in schizophrenia

Fig. 1

A Diagram of the main stages of the study. DoD: day of differentiation. This figure was created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com. B Gene expression analysis by qPCR of NPC (PAX6, NES) and astrocyte-specific markers (GFAP, S100B, SLC1A2, SLC1A3, FABP7, AQP4, ALDH1L1, ALDOC) in SCZ (red bars) and CTRL cultures (blue bars). Data of triplicate measurements of five independent donors per group are presented as mean ± SEM. C Cells from day 40 cultures stained positive for the key astrocyte markers GFAP, S100B, and AQP4. Images of 2 representative CTRLs and 2 donors with SCZ out of 5 are presented. Scale bar: 200 μm. D Normalized gene expression patterns of two housekeeping genes (GAPDH and RPLP0) and specific markers of astrocyte differentiation. Confidence interval: 0.5. E Principal component analysis (PCA) showing clustering of samples across the first two principal components (PC) based on the top 1000 genes with most variance between samples.

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