Fig. 1: PAE mice show increased anxiety and corticosterone in the plasma. | Translational Psychiatry

Fig. 1: PAE mice show increased anxiety and corticosterone in the plasma.

From: Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders

Fig. 1

A Experimental timeline. B–D PAE mice show a significant decrease in total time (B, p = 0.0015), frequency (C, p = 0.0034), and duration (D, p = 0.0057) of self-grooming compared to control mice. n = 14 per group. E Representative images of line tracks generated by MouBeat ImageJ plug-in from a recording of a mouse in an EPM test. Dashed and solid boxes outline open and closed arms, respectively. F–H Time in open arm (F, p = 0.0259), the number of entries to open arm (G, p = 0.0580), and total travel distance (H, p = 0.0059) are significantly reduced in the PAE mice. n = 9 per group. I Fold change of the plasma corticosterone (CORT) concentration was compared to the naïve control group (control mice that were not placed in any behavior tests). In both naïve conditions and 60 min post EPM test, PAE mice show a significant increase in plasma CORT level compared to the control mice (p = 0.0332 and p = 0.0192, respectively). Naïve: Control n = 12, PAE n = 9, EPM: Control n = 10, PAE n = 9. J A correlation matrix is presented to show associations between variables of behavior tests (self-grooming and EPM) and concentrations of plasma CORT. *,**,*** = p < 0.05, 0.01, 0.001 by one-tailed Pearson’s correlation coefficient. B–D, F–I Graphs represent mean ± SEM, and each dot represents an individual animal. Unpaired two-tailed Student’s t-test was used for statistical analyses. *,**p < 0.05, 0.01.

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